Introduction: Tree shrew is considered to be the most approximate mammal with primate. At the same time, it is considered to have the most potential to take the place of mammal for human disease research.This research gives a test about the αGal antigen in tree shrew to see its potential on development of xenotransplantation model research.

Materials and Methods: This research use FCM analyses αGal antigen in tree shrew cells matched with rat various visceral organ cells, HUVEC, porcine endothelial cell and macaque endothelial cells. First of all,we hocus the tree shrew with 10% chloral hydrate at the dose of 4.5g/Kg and then we take out the heart, liver, spleen, lung and kidney from tree shrew. Grind them into pieces in the base of Red Blood Cell Lysis Buffer separately. Digest them into single cells by CollagenaseⅡ and pancreatic enzymes and then turn these cells into RPMI 1640 medium. These cells are set as control group. The matched Group1 is designed as cells of heart, liver, spleen, lung, kidney from rat in the same preparation method of control group.The matched Group2 is designed as HUVEC, porcine endothelial cell and macaque endothelial cells which are bought by research laboratory.

Results: The control group showed that cells from heart, liver, lung, kidney are αGal weak positive and cells from spleen are αGal strong positive. Group1: cells from heart, spleen, lung, kidney of rat are αGal strong positive, and cells from liver of rat are weakly positive. Group2:porcine endothelial cells are αGal strong positive, HUVEC and macaque endothelial cells areαGal negative.

Discussion: Cells from tree shrew express αGal antigen. With the development of αGal gene knockout technology more and more popular, it is easy to set up a xenotransplantation model which makes rat donor and tree shrew receptor.This model can avoid the interference of anti-Gal antibody so to reduce the cost.Tree shrew is small shaped,low cost breeding and easy to operate thus the most ideal animal of choice for xenotransplantation model.