Aim: To establish a novel liver auto-transplantation pig model supported by venous bypass and evaluate effect of hypothermic machine perfusion on liver underwent warm ischemia injury.

Method: 10 Landrace pigs were used to establish standardized auto-liver transplantation models (CS model group). Another 10 Landrace pigs were randomized into two groups after 30mins of warm ischemia, in one group, pig livers were preserved with cold storage (WI+CS) and another group underwent hypothermic machine perfusion (WI+HMP) before auto-transplantation. Blood samples of different time points were collected for biochemical analysis and animal survival rate was analyzed after auto-transplantation.

Results: The animals in the CS model group all survived over 30 days after the post liver auto-transplantation, which had significant differences with WI+CS group (P<0.001) and WI+HMP group (P<0.001). 24h and 48h survival rates of the WI+CS group were 0 and 80%, 0 and 20% for WI+HMP group. As a result, hypothermic perfusion prolonged the pig’s survival time which undertook post liver auto-transplantation with 30min warm ischemia injury (P=0.014). HMP had decreased the bilirubin level at 4h post transplantation of warm ischemia liver compared with CS (P=0.014).

Conclusion: Venous bypass supporting pig liver auto-transplantation is a stable model for evaluation of liver functions. Hypothermic machine perfusion can improve warm ischemic animal’s survival rate.